a new primer pair in its1 region for molecular studies on echinococcus granulosus

background: echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. the strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. using dna based methods for strain /genotype characterizations of e. granulosus have some difficulties, especially access to an efficient and pure concentration of dna and proper primers. methods: using grinder method, a pure and high concentration dna was extracted from 10 human hydatid cysts collected from isfahan (central iran) hospitals, and processed for pcr reaction. results: using dnasis, the primers were designed in internal transcribed spacer 1 (its1) region, following analysis of 30 e. granulosus nucleotide sequences, extracted from gene bank. conclusion: this new and specific e. granulosus primer which amplified dna thoroughly can be applied for molecular studies on echinococcosis.